scaled or arcsinh-transformed expression for annotation?
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Thank you for developing this excellent tool.
In the manuscript, it suggested to use the heatmap from Figure 6 to annotate clusters, but we have a question regarding the use of scaled expression values (0-1). Since this scaling can artificially inflate low-expression markers, would using arcsinh-transformed values be a more appropriate method for cluster annotation?
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Start with the manuscript’s discussion of Figure 6 and the heatmap guidance for cluster annotation. Compare the stated use of scaled expression values with the question about arcsinh-transformed values; done would be a resolved, documented recommendation, but the issue names no implementation file or test.
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