scverse / scverse/spatialdata-plot
Q: How to render xenium morphology image with original colors?
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描述
Hello @LucaMarconato!
Thank you very much for this tool. I want to plot a part of morphology image acquired with xenium + multimodal segmentation kit. It is loaded into spatialdata and plotted without errors, but the colors are dim and they are different from what I'd expected (DAPI - #0F73E6, Boundary - #F300A5, Interior RNA - #A4A400, interior protein - #008A00, reference values from Xenium Explorer). I tried passing a list of colors like this:
crop = lambda sdata: spatialdata.bounding_box_query(
sdata,
min_coordinate=[17_500, 55_000],
max_coordinate=[19_500, 57_000],
axes=("x", "y"),
target_coordinate_system="global",
)
crop(sdata).pl.render_images("morphology_focus", ["#0F73E6", "#F300A5", "#A4A400", "#008A00"]).pl.show(
ax=axes[0], title="Morphology image", coordinate_systems="global"
)
The code does not produce an error, it works twice as long, and it results in the same image with colors unchanged.
The key question is how to adjust the colors and saturation of the plot, so that it looked closer to what I see in Xenium Explorer?
Secondary question is how to translate physical coordinates into spatialdata's global coordinate system? Am I supposed to get transformation and then apply transformation to a single or a couple points to get min and max for cropping above? I can make a separate issue with this question, if necessary.
Best,
Vasily
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调研方向
从 morphology_focus 上的 render_images 调用开始,跟踪其颜色参数如何影响绘制的图像。将绘制出的颜色与 Xenium Explorer 的参考值进行比较,并分别检查 bounding_box_query 如何对提供的物理坐标使用全局坐标系。完成的标准是:颜色行为和坐标转换已通过可复现示例记录或修正。
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