AllenNeuralDynamics / AllenNeuralDynamics/brainwasher

Minor Cross-Contamination from slight "Backwash"

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#7 1 comentario 0 reacciones 1 asignado Reclamado por @Poofjunior Ver en GitHub
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Python
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0
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1
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Descripción

Running a protocol produces small droplets as "backwash" into some lines.

![pressure_eq_bug](https://github.com/user-attachments/assets/9e38e032-137d-4984-8c23-bb2b722788c9)

This issue has to do with switching between chemicals on the rotary shear valve while having a difference in pressure between the chemical source line and the pump. It could also be an artifact due to the dead volume of gas (i.e: compressible) between the syringe and the rotary shear valve.

There are a few approaches before we can consider this "fixed."
* If we can prevent the primed line from moving altogether when we switch ports on the rotary shear valve, that's ideal.
* If we can remove the residual liquid such that there is no cross contamination but the actual primed line moves slightly, that's ok too.

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