ome / ome/ngff

Thickness of 2D slice

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Python
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Description

For some microscope modalities one collects 2D images, but it can make sense to still assign them a physical thickness along axis perpendicular to the 2D plane, e.g the axial extend of the PSF in light microscopy or the section thickness in TEM.

Such a thickness is interesting for analysis but also for rendering, e.g. when stacking many slices and then looking at them from the side.

How could we represent this in OME-Zarr? Would we store the data as 3D with the 3rd dimension having a length of one in dataspace?

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Research direction

Start by reviewing the OME-Zarr specification's existing conventions for 2D and 3D dataspace representations. Compare a singleton third dimension with a separate physical-thickness representation for analysis and side-view rendering. Done means the representation and its implications are agreed and documented.

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Assessment

Domain
data
Issue type
Feature
Difficulty
5/5
Estimated time
Over a week
Activity status
Quiet
Clarity
Needs clarification
Newbie friendliness
35/100

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