nf-core / nf-core/website

advisory for nf-core/ampliseq is missing incompatible Nextflow version

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@mashehu is already working on this.

Since Apr 7, 2026.

bug
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Description

Describe the bug
nf-core/ampliseq does not run with Nextflow version 25.10.2 and produces a /bin/bash: line 10: .command.run: Permission denied error. There is an advisory on the website that describes this issue, but it limits the problem to Nextflow versions 25.04.0-25.04.3.

Error message

 Workflow execution completed unsuccessfully

The exit status of the task that caused the workflow execution to fail was: 126

Error executing process > 'NFCORE_AMPLISEQ:AMPLISEQ:FORMAT_TAXONOMY'

Caused by:
  Process `NFCORE_AMPLISEQ:AMPLISEQ:FORMAT_TAXONOMY` terminated with an error exit status (126)


Command executed:

  taxref_reformat_standard.sh \
  
  #Giving out information
  echo -e "--dada_ref_taxonomy: silva=138\n" >ref_taxonomy.silva_138.txt
  echo -e "Title: Silva 138.1 prokaryotic SSU\n" >>ref_taxonomy.silva_138.txt
  echo -e "Citation: Quast C, Pruesse E, Yilmaz P, Gerken J, Schweer T, Yarza P, Peplies J, Glöckner FO. The SILVA ribosomal RNA gene database project: improved data processing and web-based tools. Nucleic Acids Res. 2013 Jan;41(Database issue):D590-6. doi: 10.1093/nar/gks1219. Epub 2012 Nov 28. PMID: 23193283; PMCID: PMC3531112.\n" >>ref_taxonomy.silva_138.txt
  echo "All entries: [title:Silva 138.1 prokaryotic SSU, file:[/proj/ngi2016003/nobackup/chuan/running_version/ampliseq-DB/silva_nr99_v138.1_train_set.fa.gz, /proj/ngi2016003/nobackup/chuan/running_version/ampliseq-DB/silva_species_assignment_v138.1.fa.gz], citation:Quast C, Pruesse E, Yilmaz P, Gerken J, Schweer T, Yarza P, Peplies J, Glöckner FO. The SILVA ribosomal RNA gene database project: improved data processing and web-based tools. Nucleic Acids Res. 2013 Jan;41(Database issue):D590-6. doi: 10.1093/nar/gks1219. Epub 2012 Nov 28. PMID: 23193283; PMCID: PMC3531112., fmtscript:taxref_reformat_standard.sh, dbversion:SILVA v138.1 (https://zenodo.org/record/4587955)]" >>ref_taxonomy.silva_138.txt
  
  cat <<-END_VERSIONS > versions.yml
  "NFCORE_AMPLISEQ:AMPLISEQ:FORMAT_TAXONOMY":
      bash: $(bash --version | sed -n 1p | grep -Eo 'version [[:alnum:].]+' | sed 's/version //')
  END_VERSIONS

Command exit status:
  126

Command output:
  (empty)

Command error:
  INFO:    Environment variable SINGULARITYENV_TMPDIR is set, but APPTAINERENV_TMPDIR is preferred
  INFO:    Environment variable SINGULARITYENV_NXF_TASK_WORKDIR is set, but APPTAINERENV_NXF_TASK_WORKDIR is preferred
  INFO:    Environment variable SINGULARITYENV_NXF_DEBUG is set, but APPTAINERENV_NXF_DEBUG is preferred
  INFO:    Environment variable SINGULARITYENV_SNIC_TMP is set, but APPTAINERENV_SNIC_TMP is preferred
  /bin/bash: line 10: .command.run: Permission denied

Work dir:
  /vulpes/proj/ngis/ngi2016003/nobackup/NGI/ANALYSIS/P38402/ampliseq_analysis/work/3a/71a4d1d8be970b18a6faea96288dc8

Container:
  /vulpes/ngi/containers/biocontainers/containers.biocontainers.pro-s3-SingImgsRepo-biocontainers-v1.2.0_cv1-biocontainers_v1.2.0_cv1.img.img

Tip: you can replicate the issue by changing to the process work dir and entering the command `bash .command.run`

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