nf-core / nf-core/drop

Add NCBI hg38 and hg19 BSgenomes to the containers

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bug
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Description

Description of the bug

Currently the containers used for all Fraser and SpliceCounts modules don't include NCBI hg38 and hg19 support. This should be added since the pipeline does support those references. This currently causes the following error:

  Error in .stopOnAvailablePkg(genome) :
    BSgenome.Hsapiens.NCBI.GRCh38 package is not currently installed.
    You first need to install it, which you can do with:
        library(BiocManager)
        install("BSgenome.Hsapiens.NCBI.GRCh38")
  Calls: getBSgenome ... .getInstalledPkgnameFromGenome -> .stopOnAvailablePkg
  Execution halted
Command used and terminal output

Relevant files

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System information

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First steps

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  2. Comment on the issue to say you are picking it up — it saves two people doing the same work.
  3. Fork the repository and make your change on a branch.
  4. Open a pull request that references the issue number.

Research direction

Locate the container definitions used by the Fraser and SpliceCounts modules, then review how installed R/Bioconductor packages are specified. Confirm that the NCBI hg38 and hg19 BSgenome packages are available in both containers and verify the pipeline can use those references without the reported missing-package error.

Written by the indexing model from the issue text.

Assessment

Tech stack
r
Domain
bioinformatics
Issue type
Bug
Difficulty
3/5
Estimated time
1-2 days
Activity status
Quiet
Clarity
Mostly clear
Newbie friendliness
58/100

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