compare multiple pairs concurrently

Hi,

My dataset contains single-cell data with the following 6 treatments:

A
A+B
A+B+C
A+C
A+C+D
A+B+C+D

I would like to know the genes specifically induced by B. I wonder if it is okay and more robust to perform pairwise comparisons as below and get the aggregated output of significant genes induced by B in all conditions?
A vs A+**B**
A+C vs A+C+**B**
A+C+D vs A+C+D+**B**

Thanks!