biocore / biocore/emp

Sequences dropped by split libraries

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Dominant language
Jupyter Notebook
Stars
169
Forks
68
PR merge metrics
No merged PRs in 30d

Description

Check drop of sequences at the split library stage and if that has any correlation with env or something else.

The easiest that comes to mind to do this is to change params in split libraries to only discard sequences based on barcode and then compare the numbers per sample ...

Contributor guide

No contributing guide indexed for this repository

Research direction

Start by reproducing the split library stage and compare sequence counts per sample when filtering is limited to barcode-based discards. Determine whether sequence loss correlates with the environment or another factor, and document the observed counts and correlation.

Written by the indexing model from the issue text.

Assessment

Tech stack
jupyter-notebook
Domain
bioinformatics
Issue type
Bug
Difficulty
4/5
Estimated time
3-5 days
Activity status
Stale
Clarity
Needs clarification
Newbie friendliness
35/100

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