bedapub / bedapub/splicekit

Inquiry About Integrating RBP Binding and Knockdown RNA-seq in Splicekit

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Description

Dear Splicekit Developers,

I was wondering whether Splicekit supports the integration of RBP binding data (e.g., CLIP-seq peaks) with RNA-seq data from RBP knockdown experiments to investigate differential alternative splicing. Specifically, can Splicekit be used to assess whether RBP binding near splice sites directly influences their usage upon knockdown?

I’m aware that rMAPS2 offers similar functionality, but its source code is not publicly available and appears not to have been updated since 2020.

Thank you in advance for your time and help.

Best regards,
Xiao

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