AllenNeuralDynamics / AllenNeuralDynamics/aind-hcr-pairwise-unmixing-calibrated

Fix class thresholding

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Langage dominant
Python
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0
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Merge moyen
1 h 9 min
PR mergées (30 j)
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Description

the threshold of 100 transcripts to call a cell positive for Slc17a7 is probably too high. either Slc17a7 spots need to be thresholded for intensity, as was done in prior versions, or we should increase the threshold Currently cells that are high for Gad2 and Slc17a7, of which there are many, are labeled as unassigned even though most of them are obviously inhibitory. The definition of inhibitory should be high for any subclass gene or Gad2, regardless of the Slc17a7 expression.

Could also consider using GMM for this

Guide de contribution

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Piste de recherche

Locate the current cell-labeling code that applies the Slc17a7 and Gad2 thresholds, then compare it with the prior-version intensity thresholding if that implementation is available. Clarify whether the fix should use intensity thresholding, a higher threshold, or GMM; done means cells high for Gad2 or any subclass gene are classified as inhibitory rather than unassigned, with the chosen behavior validated on the spot tables.

Rédigé par le modèle d'indexation à partir du texte de l'issue.

Évaluation

Stack technique
python
Domaine
data, machine-learning
Type d'issue
Bug
Difficulté
4/5
Temps estimé
3-5 jours
Activité
Active
Clarté
À clarifier
Accessibilité débutants
38/100

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