sokrypton / sokrypton/ColabFold
Missing uniref30_2302_db_aln when running colabfold_search with local MMseqs2
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Description
Body:
Hi ColabFold/MMseqs team,
I'm encountering an error when running colabfold_search with local MMseqs2 databases. Here's the command I'm using:
colabfold_search \
--mmseqs /home/junyu/project/mmseqs2/mmseqs/bin/mmseqs \
/home/junyu/project/sym/motif_sym/paper_design/c/c3/MPNN_results/seqs/C3_idx_0_len_240.fa \
/media/junyu/WinDATA/colab_database \
msas
However, the program crashes with the following error:
Input /media/junyu/WinDATA/colab_database/uniref30_2302_db_aln does not exist
Traceback (most recent call last):
File ".../colabfold_search", line 8, in <module>
sys.exit(main())
...
File ".../search.py", line 94, in mmseqs_search_monomer
run_mmseqs(mmseqs, ["expandaln", ..., dbbase.joinpath(f"{uniref_db}{dbSuffix2}"), ...])
...
subprocess.CalledProcessError: Command '[... 'expandaln', ..., '/uniref30_2302_db_aln', ...]' returned non-zero exit status 1.
I checked my database folder at /media/junyu/WinDATA/colab_database/ and indeed, there is no file named uniref30_2302_db_aln.
❓Questions
- Should this
_alnfile be generated bysetup_databases.sh? - If not, where can I obtain it or how should I generate it from
uniref30_2302.tar.gz?
Contributor guide
First steps
- Read the whole issue, then the project's contributing guide.
- Comment on the issue to say you are picking it up — it saves two people doing the same work.
- Fork the repository and make your change on a branch.
- Open a pull request that references the issue number.
Research direction
Start with setup_databases.sh and the mmseqs_search_monomer call in search.py around line 94. Check which database artifacts the script expects from uniref30_2302.tar.gz and compare them with the command's colab_database directory. Done means the required _aln artifact is available or the expected setup path is documented and colabfold_search proceeds past expandaln.
Written by the indexing model from the issue text.
Assessment
- Tech stack
- bash
- Domain
- bioinformatics, databases
- Issue type
- Bug
- Difficulty
- 3/5
- Estimated time
- 1-2 days
- Activity status
- Stale
- Clarity
- Needs clarification
- Newbie friendliness
- 28/100