sokrypton / sokrypton/ColabFold

prediction error using alphafold multimer

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Jupyter Notebook
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Description

Hi,

I'm designing antibody for my protein using RFdiffusion and ProteinMPNN.
subsequently, I'm predicting the structure of the sequence obtained from proteinMPNN using colabfold alphafold multimer.

I have a question about the structure obtained from colabfold alphafold multimer.

first, my protein is dimer. so, I predict that, designed antibody interacts only one protomer of dimer.
however, designed antibody seems to combine (interact) center of the dimer.
I think the antibody is slightly shifted.

Is there any solution?
What can I try?

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Research direction

Review the reported RFdiffusion → ProteinMPNN → ColabFold AlphaFold Multimer workflow and reproduce the dimer/antibody prediction described in the issue. A useful resolution would identify whether the central binding geometry is expected or a reproducible prediction problem and document a concrete setting or workflow to try; the issue provides no sequence, notebook, or test.

Written by the indexing model from the issue text.

Assessment

Tech stack
jupyter-notebook
Domain
bioinformatics
Issue type
Bug
Difficulty
5/5
Estimated time
Over a week
Activity status
Stale
Clarity
Needs clarification
Newbie friendliness
15/100

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