sokrypton / sokrypton/ColabFold
Generating MSAs for chimeric proteins
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Description
I'm attempting to generate predictions for multimeric complexes, where one chain is a chimeric/fusion protein and has components from different organisms. About ~85% of this chain is from one organism and the other ~15% is from another organism. The sequence coverage plot shows virtually 0 sequences aligning to the latter (and the predicted structure is very low confidence), likely due to this 15% component comprising such a small part of the overall protein that the sequences that align score very low in the search and are omitted. Is there any way to 'lock' segments of a single protein (especially chimeric proteins) to ensure diversity of sequences across that chain if the chain has components from different lineages?
Alternatively, if I need to create a custom MSA, is there a way to extract a .a3m file from ColabFold for the sequences that were used as the input (those shown in the sequence coverage plot)? All of the .a3m files that I can access seem to contain ~20k sequences, whereas the coverage plot only shows the final ~3k that went through the final seq diversity filtering.
Many thanks in advance!
Contributor guide
First steps
- Read the whole issue, then the project's contributing guide.
- Comment on the issue to say you are picking it up — it saves two people doing the same work.
- Fork the repository and make your change on a branch.
- Open a pull request that references the issue number.
Research direction
Start with ColabFold's sequence coverage plot and the accessible .a3m outputs, comparing their ~20k sequences with the ~3k sequences retained after final sequence-diversity filtering. Determine whether segment locking for chimeric proteins or extraction of the filtered MSA is supported; done requires a documented, reproducible workflow or a clearly scoped implementation path.
Written by the indexing model from the issue text.
Assessment
- Tech stack
- jupyter-notebook
- Domain
- bioinformatics
- Issue type
- Feature
- Difficulty
- 5/5
- Estimated time
- Over a week
- Activity status
- Stale
- Clarity
- Needs clarification
- Newbie friendliness
- 25/100