NotImplementedError: A UTF-8 locale is required. Got ANSI_X3.4-1968

Expected Behavior

I am trying to predict structures and include Amber refinement for more accurate side chain positioning.

Current Behavior

When running Alphafold2_advanced.ipynb with Amber enabled (step 6) downloading results fails.

Steps to Reproduce (for bugs)

Notebook run normally using standard setting, or altering any other setting (i.e., no problem downloading structures). I get this error whenever Amber is enabled from new or existing connections to Colab servers.

ColabFold Output (for bugs)

NotImplementedError Traceback (most recent call last)
in ()
51
52 # --- Download the predictions ---
---> 53 get_ipython().magic('shell zip -FSr {I["output_dir"]}.zip {I["output_dir"]}')
54 if IN_COLAB:
55 files.download(f'{I["output_dir"]}.zip')

3 frames
/usr/local/lib/python3.7/dist-packages/IPython/core/interactiveshell.py in magic(self, arg_s)
2158 magic_name, _, magic_arg_s = arg_s.partition(' ')
2159 magic_name = magic_name.lstrip(prefilter.ESC_MAGIC)
-> 2160 return self.run_line_magic(magic_name, magic_arg_s)
2161
2162 #-------------------------------------------------------------------------

/usr/local/lib/python3.7/dist-packages/IPython/core/interactiveshell.py in run_line_magic(self, magic_name, line)
2079 kwargs['local_ns'] = sys._getframe(stack_depth).f_locals
2080 with self.builtin_trap:
-> 2081 result = fn(*args,**kwargs)
2082 return result
2083

/usr/local/lib/python3.7/dist-packages/google/colab/_system_commands.py in _shell_line_magic(line)
68 exit code.
69 """
---> 70 result = _run_command(line, clear_streamed_output=False)
71 result.check_returncode()
72 return result

/usr/local/lib/python3.7/dist-packages/google/colab/_system_commands.py in _run_command(cmd, clear_streamed_output)
166 if locale_encoding != _ENCODING:
167 raise NotImplementedError(
--> 168 'A UTF-8 locale is required. Got {}'.format(locale_encoding))
169
170 parent_pty, child_pty = pty.openpty()

NotImplementedError: A UTF-8 locale is required. Got ANSI_X3.4-1968

Your Environment

I am using AlphaFold2_advanced.ipynb on Google Colab website.

Open Alphafold2_advanced.ipynb and inspect step 6, especially the shell zip command used to package output. Reproduce with Amber enabled in Google Colab and compare the locale seen by that command with the reported ANSI_X3.4-1968 error. Done when downloading results works with Amber enabled, or the notebook documents a supported workaround.