sokrypton / sokrypton/ColabFold

Indexdb died error message when creating colabfold_envdb_202108_db with MMseqs

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Description

Hi,

I was trying to setup the database.
But it breaks upon the execution of this code:

mmseqs createindex colabfold_envdb_202108_db tmp2 --remove-tmp-files 1
The error message I get is this:

MMseqs Version:          	edb8223d1ea07385ffe63d4f103af0eb12b2058e
Seed substitution matrix 	aa:VTML80.out,nucl:nucleotide.out
k-mer length             	0
Alphabet size            	aa:21,nucl:5
Compositional bias       	1
Max sequence length      	65535
Max results per query    	300
Mask residues            	1
Mask lower case residues 	0
Spaced k-mers            	1
Spaced k-mer pattern
Sensitivity              	7.5
k-score                  	seq:0,prof:0
Check compatible         	0
Search type              	0
Split database           	0
Split memory limit       	0
Verbosity                	3
Threads                  	8
Min codons in orf        	30
Max codons in length     	32734
Max orf gaps             	2147483647
Contig start mode        	2
Contig end mode          	2
Orf start mode           	1
Forward frames           	1,2,3
Reverse frames           	1,2,3
Translation table        	1
Translate orf            	0
Use all table starts     	false
Offset of numeric ids    	0
Create lookup            	0
Compressed               	0
Add orf stop             	false
Overlap between sequences	0
Sequence split mode      	1
Header split mode        	0
Strand selection         	1
Remove temporary files   	true

indexdb colabfold_envdb_202108_db colabfold_envdb_202108_db --seed-sub-mat aa:VTML80.out,nucl:nucleotide.out -k 0 --alph-size aa:21,nucl:5 --comp-bias-corr 1 --max-seq-len 65535 --max-seqs 300 --mask 1 --mask-lower-case 0 --spaced-kmer-mode 1 -s 7.5 --k-score seq:0,prof:0 --check-compatible 0 --search-type 0 --split 0 --split-memory-limit 0 -v 3 --threads 8

Target split mode. Searching through 34 splits
Estimated memory consumption: 29G
Write VERSION (0)
Write META (1)
Write SCOREMATRIX3MER (4)
Write SCOREMATRIX2MER (3)
Write SCOREMATRIXNAME (2)
Write SPACEDPATTERN (23)
Write GENERATOR (22)
Write DBR1INDEX (5)
Write DBR1DATA (6)
Write DBR2INDEX (7)
Killed
Error: indexdb died

It works fine with uniref30_2103.tar.gz file though.

How can I resolve the problem?

G.V.

Contributor guide

Open the contributing guide

First steps

  1. Read the whole issue, then the project's contributing guide.
  2. Comment on the issue to say you are picking it up — it saves two people doing the same work.
  3. Fork the repository and make your change on a branch.
  4. Open a pull request that references the issue number.

Research direction

Start with the reported mmseqs createindex colabfold_envdb_202108_db tmp2 --remove-tmp-files 1 command and its output, especially the estimated memory and Killed line. Compare the failing database setup with uniref30_2103.tar.gz, and verify the environment and resource limits used. Done means the cause and a verified resolution are recorded.

Written by the indexing model from the issue text.

Assessment

Domain
bioinformatics, databases
Issue type
Bug
Difficulty
4/5
Estimated time
3-5 days
Activity status
Stale
Clarity
Mostly clear
Newbie friendliness
25/100

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