Missing filtered table in polygon_query() of MERFISH data
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Description
Using this tutorial for MERFISH data, I found that the output of polygon_query(sdata, my_napari_annotation) does not contain any filtered table, despite my_napari_annotation covering multiple cells. Is there something missing in my code?
With filter_table=False, I get the unfiltered table, which is not what I am looking for. This is similar to https://github.com/scverse/spatialdata/issues/532
Much appreciated!
Details:
# load
sdata = sio.merscope(full_paths, backend="rioxarray", slide_name="s1", region_name="r0")
sdata
SpatialData object
βββ Points
β βββ 's1_r0_transcripts': DataFrame with shape: (<Delayed>, 9) (2D points)
βββ Shapes
β βββ 's1_r0_polygons': GeoDataFrame shape: (96632, 9) (2D shapes)
βββ Tables
βββ 'table': AnnData (96632, 500)
with coordinate systems:
βΈ 'global', with elements:
s1_r0_transcripts (Points), s1_r0_polygons (Shapes)
sdata.table.uns
OrderedDict([('spatialdata_attrs',
{'region': 's1_r0_polygons',
'region_key': 'cells_region',
'instance_key': 'EntityID'})])
sdata["table"]
AnnData object with n_obs Γ n_vars = 96632 Γ 500
obs: 'fov', 'volume', 'center_x', 'center_y', 'min_x', 'min_y', 'max_x', 'max_y', 'anisotropy', 'transcript_count', 'perimeter_area_ratio', 'solidity', 'DAPI_raw', 'DAPI_high_pass', 'PolyT_raw', 'PolyT_high_pass', 'region', 'slide', 'dataset_id', 'cells_region', 'EntityID'
uns: 'spatialdata_attrs'
obsm: 'blank', 'spatial'
print(sd.SpatialData.get_annotated_regions(sdata["table"]))
s1_r0_polygons
# annotate shape
interactive = napari_spatialdata.Interactive(sdata)
interactive.run()
# export shape layer from Napari
shape = "overlap"
sdata[shape]
geometry
0 POLYGON ((5423.45 1777.948, 6398.625 1777.948,...
sdata[shape].geometry.iloc[0]
sdata
SpatialData object
βββ Points
β βββ 's1_r0_transcripts': DataFrame with shape: (<Delayed>, 9) (2D points)
βββ Shapes
β βββ 'overlap': GeoDataFrame shape: (1, 1) (2D shapes)
β βββ 's1_r0_polygons': GeoDataFrame shape: (96632, 9) (2D shapes)
βββ Tables
βββ 'table': AnnData (96632, 500)
with coordinate systems:
βΈ 'global', with elements:
s1_r0_transcripts (Points), overlap (Shapes), s1_r0_polygons (Shapes)
# sd.polygon_query
subset = sd.polygon_query(
sdata, polygon=sdata[shape].geometry.iloc[0], target_coordinate_system="global", filter_table=True
)
subset
SpatialData object
βββ Shapes
βββ 'overlap': GeoDataFrame shape: (1, 1) (2D shapes)
with coordinate systems:
βΈ 'global', with elements:
overlap (Shapes)
# subset does not contain filtered table
Contributor guide
First steps
- Read the whole issue, then the project's contributing guide.
- Comment on the issue to say you are picking it up β it saves two people doing the same work.
- Fork the repository and make your change on a branch.
- Open a pull request that references the issue number.
Research direction
Reproduce the documented MERFISH workflow and the shown polygon_query() call with filter_table=True, then compare it with filter_table=False and review the related issue 532. Done means the resulting SpatialData object includes the filtered table for the annotated polygon.
Written by the indexing model from the issue text.
Assessment
- Tech stack
- python
- Domain
- data
- Issue type
- Bug
- Difficulty
- 3/5
- Estimated time
- 1-2 days
- Activity status
- Stale
- Clarity
- Mostly clear
- Newbie friendliness
- 38/100