scverse / scverse/spatialdata-io

proposal for friendly spatialdata_io.stereoseq function

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Dominant language
Python
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103
Forks
65
Avg merge
1h 8m
Merged PRs (30d)
3

Description

Encountering an issue with missing files when reading BGI's stereo seq data, and there is an inconsistency between the output files from the count command of SAW (a tool under BGI's technology similar to 10x spaceranger) and what is read by spatialdata_io.stereoseq.

Specifically want to know if it is because the SAW output has been updated, making it incompatible with the file reading command of spatialdata_io (there is no cell.cluster file, but it exists in another analysis command of SAW output file).

My proposal is whether there can be a simple reading script (such as one that can just read the 10x Visium output directory and create zarr data) for easy reading(gef gem file).

Contributor guide

Open the contributing guide

First steps

  1. Read the whole issue, then the project's contributing guide.
  2. Comment on the issue to say you are picking it up — it saves two people doing the same work.
  3. Fork the repository and make your change on a branch.
  4. Open a pull request that references the issue number.

Research direction

Start by inspecting the spatialdata_io.stereoseq reader and comparing the files produced by SAW's count command with the files expected by the reader, especially the missing cell.cluster file. Review the alternate SAW analysis output and the proposed Visium-style directory and GEF/GEM inputs. Done requires a decided compatibility scope and a documented, working reading path.

Written by the indexing model from the issue text.

Assessment

Tech stack
python
Domain
bioinformatics, data
Issue type
Feature
Difficulty
5/5
Estimated time
Over a week
Activity status
Stale
Clarity
Needs clarification
Newbie friendliness
25/100

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