scverse / scverse/spatialdata-io

Export the real global coordinates of cells (Xenium)

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Dominant language
Python
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Description

Hi,

I am working with a Xenium dataset that includes an H&E image as well as an alignmentmatrix.csv file. I understand that this alignment matrix can be used to transform the image data into the global coordinate system.
image

I have successfully created an sdata object from this dataset.
How can I locate a specific cell within the H&E image? More specifically, now the H&E and the cell location are aligned, but how can I explicitly know the exact cell locations on the H&E image? Thanks for your time!!

image

Contributor guide

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First steps

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  2. Comment on the issue to say you are picking it up — it saves two people doing the same work.
  3. Fork the repository and make your change on a branch.
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Research direction

Start by reproducing the Xenium dataset workflow described in the issue, including the sdata object, H&E image, and alignmentmatrix.csv. Trace how cell locations and image transforms are represented, then identify the project entry point for exposing global cell coordinates; done means a documented or callable way to obtain exact cell positions in the H&E coordinate system.

Written by the indexing model from the issue text.

Assessment

Tech stack
python
Domain
bioinformatics, data
Issue type
Feature
Difficulty
4/5
Estimated time
3-5 days
Activity status
Stale
Clarity
Needs clarification
Newbie friendliness
28/100

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