feature selection method using pearson residuals discrepancies
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Description
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What happened?
Hi,
I have performed multiple way to do features selection using pearson residuals and methods return different results.
Method 1 plot:
Method 2 plot:
I dont understand why these 2 methods dont produce the same result.
Based on those graph I think the best is method 2 but would like to understand why these dont produce similar plots.
Thanks in advance for your help
Minimal code sample
### 1st method:
analytic_pearson = sc.experimental.pp.normalize_pearson_residuals(adata, inplace=False)
adata.layers["analytic_pearson_residuals"] = csr_matrix(analytic_pearson["X"])
sc.pp.highly_variable_genes(adata, layer="analytic_pearson_residuals", n_top_genes=4000)
ax = sns.scatterplot(
data=adata.var, x="means", y="dispersions", hue="highly_variable", s=5
)
## 2nd method:
fig, ax = plt.subplots(1, 1, figsize=(10, 5))
sc.experimental.pp.highly_variable_genes(
adata, flavor="pearson_residuals", n_top_genes=4000
)
ax = sns.scatterplot(
data=adata.var, x="means", y="dispersions", hue="highly_variable", s=5
)
ax.set_xscale("log")
ax.set_yscale("log")
ax.set_title("Feature selection using Pearson residuals normalisation (from highly_variable_genes flavor pearson_residuals)")
pdf.savefig(bbox_inches="tight")
Error output
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Research direction
Start by reproducing the two workflows using normalize_pearson_residuals, highly_variable_genes with the pearson_residuals flavor, and the provided plotting code. Compare the intermediate values and selection criteria, then determine whether the differing plots are expected or indicate a defect; document the explanation or add a regression test if a defect is confirmed.
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Assessment
- Tech stack
- python
- Domain
- bioinformatics
- Issue type
- Bug
- Difficulty
- 4/5
- Estimated time
- 3-5 days
- Activity status
- Stale
- Clarity
- Needs clarification
- Newbie friendliness
- 25/100