scverse / scverse/scanpy

STARsolo Matrix with Velocyto --> Anndata function

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Python
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Description

The scanpy.read_10X_mtx works well for reading in the STARsolo output matrices, which are based on the CellRanger Outputs.

However, it would be nice to have a function or modification of the read_10X_mtx function (e.g. a boolean for STARsolo velocyto) to automate inputting the velocyto matrices that STARsolo outputs and placing them in the appropriate layers. A boolean switch for filtered versus raw matrices would be a good addition as well.

Contributor guide

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First steps

  1. Read the whole issue, then the project's contributing guide.
  2. Comment on the issue to say you are picking it up — it saves two people doing the same work.
  3. Fork the repository and make your change on a branch.
  4. Open a pull request that references the issue number.

Research direction

Start by reading scanpy.read_10X_mtx and the STARsolo output matrix conventions described in the issue. Define the supported Velocyto inputs and filtered-versus-raw behavior, then verify that the resulting matrices are placed in the appropriate layers; the issue does not name tests or files to run.

Written by the indexing model from the issue text.

Assessment

Tech stack
python
Domain
bioinformatics, data
Issue type
Feature
Difficulty
4/5
Estimated time
3-5 days
Activity status
Stale
Clarity
Mostly clear
Newbie friendliness
35/100

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