scverse / scverse/PyDESeq2

[BUG] stuck on dds.deseq2()

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bug
Dominant language
Python
Stars
761
Forks
90
Avg merge
25m
Merged PRs (30d)
10

Description

Everytime I DeseqDataSet using my own data (as counts/metadata dataframes or straight from my anndata object) or using the test data on the GettingStarted docs, I then try to run dds.deseq2() and the RAM shoots up and the kernel crashes.

The parameters for DeseqDataSet in the tutorials and the current version do not match.

Reallly would've liked to use this tool :(

Contributor guide

Open the contributing guide

First steps

  1. Read the whole issue, then the project's contributing guide.
  2. Comment on the issue to say you are picking it up — it saves two people doing the same work.
  3. Fork the repository and make your change on a branch.
  4. Open a pull request that references the issue number.

Research direction

Start by reproducing the kernel crash with the test data from the GettingStarted documentation, then compare its DeseqDataSet parameters with the current version. Investigate the dds.deseq2() execution and check whether both dataframe and anndata inputs show the same memory growth. Done means the documented examples complete without exhausting RAM or crashing the kernel.

Written by the indexing model from the issue text.

Assessment

Tech stack
python
Domain
bioinformatics
Issue type
Bug
Difficulty
4/5
Estimated time
3-5 days
Activity status
Stale
Clarity
Mostly clear
Newbie friendliness
35/100

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