rrwick / rrwick/Trycycler

gfa file to visualize the final consensus fasta by Bandage

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enhancement
Dominant language
Python
Stars
348
Forks
31
PR merge metrics
No merged PRs in 30d

Description

Hi Ryan,
I am using Trycycler to assembly 90 bacterial genomes from fish guts. It is an amazing and very helpful tool. I was able to obtain circular, > 98 % completeness, and very good coverage in all the genomes after following the pipeline and polishing the assemblies by medaka. However, I'm wondering if there is an easy and proper way to obtain a gfa-like file to visualize the genomes in bandage. I would like to plot those circular chromosomes and plasmids as well as those linear ones in a bandage-like figure. I've been looking if there is any available script in the Trycycler pipeline to do this, but so far I haven't found any.

Hope you can help me.

Thank you in advance.

Arturo.

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Research direction

Start by reviewing how the Trycycler pipeline produces its final consensus FASTA and whether its existing scripts expose graph information. Determine how circular chromosomes, plasmids, and linear sequences should be represented, then verify that the resulting GFA-like output can be opened and visualized in Bandage.

Written by the indexing model from the issue text.

Assessment

Tech stack
python
Domain
bioinformatics
Issue type
Feature
Difficulty
4/5
Estimated time
3-5 days
Activity status
Stale
Clarity
Needs clarification
Newbie friendliness
25/100

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