nf-core / nf-core/viralintegration

Add MetaPhlAn 3.0 module

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enhancement
Dominant language
Python
Stars
19
Forks
11
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Description

Description of feature

Last summer, @parnaljoshi came up with the idea of using MetaPhlAn to look for viral reads in tumor data. The general approach was:

  • align tumor fastq to human genome with STAR
  • save unmapped reads
  • run unmapped reads through MetaPhlAn:
    metaphlan $out_prefix"Unmapped.out.mate1",$out_prefix"Unmapped.out.mate2" --bowtie2out metagenome.bowtie2.bz2 --add_viruses --ignore_eukaryotes --nproc 16 --input_type fastq --read_min_len 30 > "analysis/star_results/"$substring.txt

First, we need to simply add the metaphlan3 module.

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