nf-core / nf-core/test-datasets
error in gff file (rnaseq branch test-datasets/reference/genes.gff)
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Description
We found a problem in the gff file you have as test.
- rnaseq
- test-datasets/reference/genes.gff
I ensembl transcript 335 649 . + . ID=YAL069W;Parent=YAL069W;geneID=YAL069W;gene_biotype=protein_coding;gene_name=YAL069W;gene_source=ensembl;gene_version=1;p_id=P3634;transcript_biotype=protein_coding;transcript_source=ensembl;transcript_version=1;tss_id=TSS1129
I ensembl exon 335 649 . + . Parent=YAL069W;exon_id=YAL069W.1;exon_number=1;exon_version=1;gene_biotype=protein_coding;gene_name=YAL069W;gene_source=ensembl;gene_version=1;p_id=P3634;transcript_biotype=protein_coding;transcript_source=ensembl;transcript_version=1;tss_id=TSS1129
I ensembl CDS 335 649 . + 0 Parent=YAL069W;exon_number=1;gene_biotype=protein_coding;gene_name=YAL069W;gene_source=ensembl;gene_version=1;p_id=P3634;protein_id=YAL069W;protein_version=1;transcript_biotype=protein_coding;transcript_source=ensembl;transcript_version=1;tss_id=TSS1129
ID and Parent attributes of transcript features have same IDs. This is not allowed in GFF3 specifications.
We use AGAT that deals with that problem by automatically updating the parent ID to be uniq.
Using this file to test/build pipelines might be problematic. This should be updated.
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First steps
- Read the whole issue, then the project's contributing guide.
- Comment on the issue to say you are picking it up — it saves two people doing the same work.
- Fork the repository and make your change on a branch.
- Open a pull request that references the issue number.
Research direction
Inspect test-datasets/reference/genes.gff on the rnaseq branch and compare the transcript ID and Parent attributes with the GFF3 specification. Use AGAT to verify the corrected fixture no longer requires automatic parent-ID rewriting; done means the test file contains unique, valid identifiers.
Written by the indexing model from the issue text.
Assessment
- Domain
- data, testing
- Issue type
- Bug
- Difficulty
- 1/5
- Estimated time
- 1-3 hours
- Activity status
- Stale
- Clarity
- Clearly specified
- Newbie friendliness
- 25/100