FILTER column empty and no annotation output
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Description
Description of the bug
Hi,
thanks for the code!
I am running Nextflow 25.04.6 and rnavar 1.2.0. The job completes, and in the results dir I see
pipeline_info
preprocessing
reports
samtools
seq2hla
variant_calling
The variant_calling dir reports a .haplotypecaller.combined.g.vcf.gz and .haplotypecaller.combined.g.vcf.gz.tbi for each sample
The FILTER column in the resulting VCFs is empty and the INFO column does not show any VEP annotation, as far as I can see.
Can you please let me know what I am missing ?
Thanks
Command used and terminal output
The command line is as follow
nextflow run nf-core/rnavar -r 1.2.0 -c $inDIR/loc.config -profile apptainer \
-work-dir $workDIR \
--input $inDIR/samplesheet.csv \
--outdir $outPATH/$outDIR \
--fasta $GENOME \
--fasta_fai $GENOMEIDX \
--gtf $GTF_PATH \
--read_length 101 \
--aligner star \
--star_twopass true \
--star_index $STAR_INDEX \
--remove_duplicates false \
--no_intervals true \
--generate_gvcf true \
--known_indels $INDELS \
--known_indels_tbi $INDELSTBI \
--dbsnp $DBSNP \
--dbsnp_tbi $DBSNPTBI \
--snpeff_db "GRCh38.p13" \
--tools seq2hla,bcfann,vep \
--vep_cache $VEPCACHEPATH/.vep \
--vep_genome "GRCh38" \
--vep_species "homo_sapiens" \
--vep_cache_version "110"
Relevant files
No response
System information
No response
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Research direction
Reproduce the supplied Nextflow command and inspect the generated files under variant_calling, especially the combined VCF and its index. Trace the annotation and filtering steps for the listed tools, then confirm that the resulting VCF has populated FILTER and VEP INFO fields.
Written by the indexing model from the issue text.
Assessment
- Domain
- bioinformatics
- Issue type
- Bug
- Difficulty
- 4/5
- Estimated time
- 3-5 days
- Activity status
- Stale
- Clarity
- Needs clarification
- Newbie friendliness
- 25/100