nf-core / nf-core/rnavar

Add samtools mpileup as an alternative variant caller

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enhancement
Dominant language
Nextflow
Stars
61
Forks
47
Avg merge
1d 1h
Merged PRs (30d)
1

Description

Description of feature

I tried using the default variants called using GATK but they didn't seem to work as well for my purpose. On the contrary samtools mpileup worked extremely well.

Maybe it would be worthwhile adding it as an alternative calling procedure to this?

Contributor guide

Open the contributing guide

First steps

  1. Read the whole issue, then the project's contributing guide.
  2. Comment on the issue to say you are picking it up — it saves two people doing the same work.
  3. Fork the repository and make your change on a branch.
  4. Open a pull request that references the issue number.

Research direction

Start by locating the existing GATK variant-calling procedure in the rnavar pipeline and reviewing how its inputs and outputs are handled. Determine where samtools mpileup could be offered as an alternative, then verify that the pipeline can run with that procedure and produces the expected variant-calling output.

Written by the indexing model from the issue text.

Assessment

Domain
bioinformatics
Issue type
Feature
Difficulty
4/5
Estimated time
3-5 days
Activity status
Stale
Clarity
Needs clarification
Newbie friendliness
35/100

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