nf-core / nf-core/phageannotator

Running geNomad and COBRA fails because of altered FASTA sequence name

Open
#125 0 comments 0 reactions 0 assignees View on GitHub

Nobody has claimed this yet.

bug
Dominant language
Nextflow
Stars
19
Forks
8
PR merge metrics
No merged PRs in 30d

Description

Description of the bug

In the geNomad summary, contigs with a prophage have a suffix added to its name. This results in COBRA unable to find the name in the original contig FASTA file.

Command used and terminal output

Relevant files

No response

System information

No response

Contributor guide

Open the contributing guide

First steps

  1. Read the whole issue, then the project's contributing guide.
  2. Comment on the issue to say you are picking it up — it saves two people doing the same work.
  3. Fork the repository and make your change on a branch.
  4. Open a pull request that references the issue number.

Research direction

Start by reproducing the geNomad-to-COBRA handoff described in the issue and trace where FASTA sequence names are transformed. The fix is complete when contig names with prophage annotations remain resolvable by COBRA against the original FASTA records.

Written by the indexing model from the issue text.

Assessment

Domain
bioinformatics
Issue type
Bug
Difficulty
4/5
Estimated time
3-5 days
Activity status
Stale
Clarity
Needs clarification
Newbie friendliness
25/100

Get new issues in your inbox

A short digest of beginner-friendly GitHub issues.