BCL_DEMULTIPLEX swf cannot handle tarballs
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Since Apr 17, 2026.
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Description
Have you checked the docs?
Description of the bug
Description
At commit f3086dae4abcf5aa695f65e352b9293b76b693ee the BCL_DEMULTIPLEX subworkflow cannot handle tarballs. The internal module BCLCONVERT was specifically designed to handle both directory paths and tarball archives. With the introduction of MULTIQCSAV module on March 4th, the swf fails if the ch_flowcell's third argument is a tarball instead of a path.
Hence, the surround subworkflow BCL_DEMULTIPLEX limits the capabilities of module BCLCONVERT.
Error log
ERROR ~ No such file or directory: https://raw.githubusercontent.com/nf-core/test-datasets/modules/data/genomics/sarscov2/illumina/bcl/200624_A00834_0183_BHMTFYDRXX.tar.gz/*.xml
-- Check script '/<path>/subworkflows/nf-core/bcl_demultiplex/main.nf' at line: 65 or see '/<path>/.nf-test/tests/a9f9a82b17265ea90d24d15669e96ce2/meta/nextflow.log' file for more details
ERROR ~ Pipeline failed.
Line 65 is
def xml = files(file(flowcell).resolve("*.xml"), checkIfExists: true)
I am using nf-core's public SARS-CoV-2 Illumina BCL test data. The tarball's files are
├── Config
├── CopyComplete.txt
├── Data
├── InterOp
├── Recipe
├── RTA3.cfg
├── RTAComplete.txt
├── RunInfo.xml
├── RunParameters.xml
└── SampleSheet.csv
Ideal fix
Both input versions (path, tarball)
ch_flowcell = [[id:"", lane:""], samplesheet.csv, path/to/bcl/files]ch_flowcell = [[id:"", lane:""], samplesheet.csv, path/to/bcl/files.tar.gz]
should be accepted by BCL_DEMULTIPLEX.
Command used and terminal output
Relevant files
- (BCL tarball) https://raw.githubusercontent.com/nf-core/test-datasets/modules/data/genomics/sarscov2/illumina/bcl/200624_A00834_0183_BHMTFYDRXX.tar.gz
- (Samplesheet) https://raw.githubusercontent.com/nf-core/test-datasets/modules/data/genomics/sarscov2/illumina/bcl/SampleSheet.csv
- (Genome) https://raw.githubusercontent.com/nf-core/test-datasets/modules/data/genomics/sarscov2/genome/genome.fasta
System information
- Nextflow version 25.10.4
- Container engine Singularity
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