nf-core / nf-core/methylseq

Option --unmapped does not run Bismark with --unmapped argument

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bug
Dominant language
Nextflow
Stars
198
Forks
181
Avg merge
6d 17h
Merged PRs (30d)
1

Description

Description of the bug

The current code does not correctly use the argument "--un" to run Bismark to save unmapped reads when the pipeline parameter "--unmapped" is specified. Unmapped reads are therefore not saved when requested. Checking the .command.sh script in the relevant work folder, the argument is not included in the Bismark align command.

Command used and terminal output

Relevant files

No response

System information

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Contributor guide

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First steps

  1. Read the whole issue, then the project's contributing guide.
  2. Comment on the issue to say you are picking it up — it saves two people doing the same work.
  3. Fork the repository and make your change on a branch.
  4. Open a pull request that references the issue number.

Research direction

Locate the Bismark alignment entry point and the pipeline's --unmapped parameter, then inspect the generated .command.sh mentioned in the report. Verify that requesting --unmapped results in the Bismark command receiving --un and that unmapped reads are saved.

Written by the indexing model from the issue text.

Assessment

Domain
bioinformatics
Issue type
Bug
Difficulty
3/5
Estimated time
1-2 days
Activity status
Stale
Clarity
Mostly clear
Newbie friendliness
45/100

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