nf-core / nf-core/methylseq

Mapping samples to multiple reference fasta

Open
#181 15 comments 1 reaction 0 assignees View on GitHub

Nobody has claimed this yet.

dsl2 enhancement
Dominant language
Nextflow
Stars
198
Forks
181
Avg merge
6d 17h
Merged PRs (30d)
1

Description

Hi,

I was thinking of adapting this pipeline to take care of multiple reference genomes. Something like each sample would be aligned to different reference fasta file. Any votes for such a feature?

The input if given would be a csv file with sample_id, path_to_reference_fasta, path_to_sample_fastq

Cheers,
Rahul

Contributor guide

Open the contributing guide

First steps

  1. Read the whole issue, then the project's contributing guide.
  2. Comment on the issue to say you are picking it up — it saves two people doing the same work.
  3. Fork the repository and make your change on a branch.
  4. Open a pull request that references the issue number.

Research direction

Start with issue #181 and inspect how the methylseq pipeline currently accepts sample and reference inputs. Consider the proposed CSV fields—sample_id, path_to_reference_fasta, and path_to_sample_fastq—and determine the scope of supporting multiple reference FASTA files per sample. Done means the requested input can drive alignment against the specified reference for each sample.

Written by the indexing model from the issue text.

Assessment

Domain
bioinformatics
Issue type
Feature
Difficulty
5/5
Estimated time
Over a week
Activity status
Stale
Clarity
Needs clarification
Newbie friendliness
25/100

Get new issues in your inbox

A short digest of beginner-friendly GitHub issues.