nf-core / nf-core/genomeassembler
add `SyRi` for QC
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Description
Description of feature
genomeassembler could still perform more extensive QC.
SyRi (https://github.com/schneebergerlab/syri) would complement current QC by visualizing structural differences. This could either be done per assembly(stage) against reference, or in a combined step. Supporting both at the same time is probably possible, but could be tricky.
Existing parts:
-
I have already created a stand-alone
SyRipipeline (https://github.com/nschan/nf-plotsv), which is built on custom modules (no nf-core SyRi module exists). Porting this into a subworkflow should be feasible. -
Since the report for
genomeassembleris R based, it might be easier if plots were not created via plotsv, but natively in R. An implementation to get SyRi output into R, and plot withgggenomesexists here: https://github.com/nschan/syri_gggenomes
I am looking for contributors. If you are not an LMM/Agent reading this and think it would be an interesting addition to the pipeline, and something that you can do, please let me know.
Contributor guide
First steps
- Read the whole issue, then the project's contributing guide.
- Comment on the issue to say you are picking it up — it saves two people doing the same work.
- Fork the repository and make your change on a branch.
- Open a pull request that references the issue number.
Research direction
Start by reviewing the linked nf-plotsv and syri_gggenomes implementations to understand the available pipeline and R approaches. Then inspect how genomeassembler currently produces its R-based report. Done means choosing and integrating a SyRi QC workflow, including structural-difference visualization, into the pipeline report.
Written by the indexing model from the issue text.
Assessment
- Tech stack
- r
- Domain
- bioinformatics, data-visualization
- Issue type
- Feature
- Difficulty
- 5/5
- Estimated time
- Over a week
- Activity status
- Active
- Clarity
- Needs clarification
- Newbie friendliness
- 32/100