nf-core / nf-core/genomeannotator
Pipeline fail at REPEATMASKER_STAGELIB
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Description
Description of the bug
I am running the most current version of @marchoeppner dev branch on Tower on a real dataset. The workflow passed repeat masking and produced the consensi.fa file but failed very quickly at the subsequent staging process with:
cp: can't stat '/.nextflow/assets/marchoeppner/genomeannotator/assets/repeatmasker/my_genome.fa': No such file or directory
Since I ran this on Tower it's a little difficult to debug things in the .nextflow/assets directory so this isn't obvious to me yet where this my_genome.fa file was supposed to come from.
Command used and terminal output
nextflow run 'https://github.com/marchoeppner/genomeannotator' \
-name tick_annotation_test \
-params-file 'https://api.tower.nf/ephemeral/zSnWhzERlz_Z4e-E6h2gng.json' \
-with-tower \
-r dev \
-profile docker
Params:
pri_prot_target = 5
custom_config_base = https://raw.githubusercontent.com/nf-core/configs/master
evm_weights = /.nextflow/assets/marchoeppner/genomeannotator/assets/evm/weights.txt
plaintext_email = false
monochrome_logs = false
pasa_nmodels = 1000
spaln_q = 5
max_cpus = 16
spaln_taxon = ixodscap
pri_rnaseq = 4
max_multiqc_email_size = 25.MB
max_time = 240.h
aug_chunk_length = 3000000
nevm = 10
tracedir = ${params.outdir}/pipeline_info
validate_params = true
npart_size = 200000000
max_intron_size = 20000
aug_species = human
pri_wiggle = 2
version = false
spaln_options = -M
publish_dir_mode = copy
aug_training = false
t_prot = P
t_est = E
pasa_config_file = /.nextflow/assets/marchoeppner/genomeannotator/assets/pasa/alignAssembly.config
min_prot_length = 35
aug_options = --alternatives-from-evidence=on --minexonintronprob=0.08 --minmeanexonintronprob=0.4 --maxtracks=3
multiqc_title = test run
pri_trans = 4
pasa = false
custom_config_version = master
max_memory = 128.GB
dummy_gff = /.nextflow/assets/marchoeppner/genomeannotator/assets/empty.gff3
spaln_protein_id_targeted = 90
nproteins = 200
proteins = s3://nf-hifi2genome/tick_annotation/2023-08-25-all-tick-species-proteins.fasta
assembly = s3://nf-hifi2genome/tick_annotation/Arcadia_Amblyomma_americanum_asm001_purged_cleanedup1.fasta
pri_est = 4
pri_prot = 3
min_contig_size = 5000
evm = false
email = elizabeth.mcdaniel@arcadiascience.com
schema_ignore_params = genomes,saveReference,igenomes_base,igenomes_ignore,enable_conda,save-reference
genomes:
pasa_aligner = minimap2
ncrna = false
igenomes_ignore = true
outdir = s3://nf-hifi2genome/tick_annotation/genomeannotator
help = false
show_hidden_params = false
t_rnaseq = E
rm_db = https://www.dfam.org/releases/Dfam_3.5/families/Dfam_curatedonly.h5.gz
busco_lineage = arthropoda_odb10
trinity = false
spaln_protein_id = 60
Relevant files
System information
Run on Tower with Nextflow version 23.04.3, using Docker profile
Contributor guide
First steps
- Read the whole issue, then the project's contributing guide.
- Comment on the issue to say you are picking it up — it saves two people doing the same work.
- Fork the repository and make your change on a branch.
- Open a pull request that references the issue number.
Research direction
Start with the linked nf-2ZIBaSqCJUU5ME.log and the REPEATMASKER_STAGELIB stage that follows repeat masking. Inspect how the repeatmasker asset path and my_genome.fa are resolved under the Docker profile and Tower execution. Done means the staging process locates the expected genome file and the workflow proceeds past this step.
Written by the indexing model from the issue text.
Assessment
- Tech stack
- docker
- Domain
- bioinformatics
- Issue type
- Bug
- Difficulty
- 4/5
- Estimated time
- 3-5 days
- Activity status
- Stale
- Clarity
- Needs clarification
- Newbie friendliness
- 25/100