nf-core / nf-core/funcscan

A uniform input for all tools in each screening workflow

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#385 2 comments 0 reactions 0 assignees View on GitHub

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enhancement
Dominant language
Nextflow
Stars
119
Forks
40
Avg merge
2d 17h
Merged PRs (30d)
5

Description

Description of feature

Currently different analysis software in ARG screening workflow use different input sequences.
For instance, AMRFinderPlus and RGI use nucleotide sequence as input whereas deeparg uses predicted protein sequences from the annotation step in the beginning of the pipeline.

Since all software accept both nucleotide and protein sequences, it would be really helpful to have a uniform input for all software which allows post-processing and comparison between predictions by different software based on sequence accession number.

https://nfcore.slack.com/archives/C02K5GX2W93/p1717515107461539

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Research direction

Start by tracing the ARG screening workflow inputs for AMRFinderPlus, RGI, and deeparg, including the annotation step that supplies predicted proteins. Determine where the workflow selects nucleotide versus protein sequences and define acceptance checks showing that every tool uses one uniform input and produces outputs comparable by sequence accession number.

Written by the indexing model from the issue text.

Assessment

Domain
bioinformatics
Issue type
Feature
Difficulty
5/5
Estimated time
Over a week
Activity status
Stale
Clarity
Needs clarification
Newbie friendliness
30/100

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