nf-core / nf-core/fetchngs

Merge technical replicates (SRR1 + SRR2 -> SRX)

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enhancement
Dominant language
Nextflow
Stars
202
Forks
93
Avg merge
1d 18m
Merged PRs (30d)
15

Description

Description of feature

The feature I'm hoping for is merging of technical replicates / runs (SRRs) within each sample/experiment (SRX/GSM/etc). I would like the fastq files to be concatenated within each index (SRR1.R1.fq + SRR2.R1.fq -> SRX.R1.fq; SRR1.R2.fq + SRR2.R2.fq -> SRX.R2.fq). Is such a feature possible in the current pipeline? This would be really nice for those who wish to go directly from fetchngs to other nf-core pipelines.

The docs suggest this should be possible (link), but it doesn't seem to be implemented yet.

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Research direction

No source files or tests are named. Start by reading the linked fetchngs usage documentation and tracing how SRR/ERR/DRR identifiers resolve to SRX/ERX/DRX experiments. Done means technical replicate FASTQ files are concatenated per index within each experiment, including both R1 and R2 outputs.

Written by the indexing model from the issue text.

Assessment

Domain
bioinformatics
Issue type
Feature
Difficulty
4/5
Estimated time
3-5 days
Activity status
Stale
Clarity
Mostly clear
Newbie friendliness
45/100

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