nf-core / nf-core/denovotranscript

Separate assemblies for separate samples

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#48 1 comment 3 reactions 0 assignees View on GitHub

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enhancement
Dominant language
Nextflow
Stars
26
Forks
3
PR merge metrics
No merged PRs in 30d

Description

Description of feature

Transferring Slack request to Github:
Currently the pipeline pools reads from all files in the samplesheet before proceeding with assembly. However, some users would find it useful to keep different samples separated and be able to generate multiple transcriptomes with only one run of the pipeline.
"I would absolutely love the pipeline to be upgraded to treat sample sheet entries with different sample names as different entries that should be kept separately, just as other nf-core pipelines such as Sarek and Rnaseq does."

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Research direction

The issue names no files, tests, or entry points. Start by tracing how samplesheet entries are grouped before assembly, then determine how separate sample names should produce independent transcriptomes in one run; done means pooled reads are no longer mixed across sample names and the resulting assemblies remain separate.

Written by the indexing model from the issue text.

Assessment

Domain
data
Issue type
Feature
Difficulty
5/5
Estimated time
Over a week
Activity status
Stale
Clarity
Mostly clear
Newbie friendliness
35/100

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