nf-core / nf-core/crisprseq

bowtie/align inconsistencies with latest NF version

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Description

bowtie alignment with latest nf version gives a different count matrix 🤯

Originally posted by @matbonfanti in https://github.com/nf-core/crisprseq/issues/263#issuecomment-3557371687

Contributor guide

Open the contributing guide

First steps

  1. Read the whole issue, then the project's contributing guide.
  2. Comment on the issue to say you are picking it up — it saves two people doing the same work.
  3. Fork the repository and make your change on a branch.
  4. Open a pull request that references the issue number.

Research direction

Begin by reproducing the bowtie alignment with the latest NF version and comparing the resulting count matrix. Check the original discussion in issue #263 for context. Done means the source of the differing counts is identified and the expected matrix behavior is verified.

Written by the indexing model from the issue text.

Assessment

Domain
bioinformatics
Issue type
Bug
Difficulty
4/5
Estimated time
3-5 days
Activity status
Stale
Clarity
Needs clarification
Newbie friendliness
25/100

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