nf-core / nf-core/crisprseq

MAGECK mle cnv correction doesn't work

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bug
Dominant language
Nextflow
Stars
63
Forks
39
PR merge metrics
No merged PRs in 30d

Description

Description of the bug

When the user adds a cnv correction with the config file, the pipeline takes ages and then fails
count_table.count.txt
Brunello_RepA_Dropout_A375_Brunello_RepB_Dropout_A375_vs_Brunello_pDNA.txt

Command used and terminal output
nextflow run crisprseq -r dev -profile cfc --count_table count_table.count.txt --mle_design_matrix Brunello_RepA_Dropout_A375_Brunello_RepB_Dropout_A375_vs_Brunello_pDNA.txt  --outdir test --analysis screening -resume -dump-channels -c user.config
Relevant files

No response

System information

No response

Contributor guide

Open the contributing guide

First steps

  1. Read the whole issue, then the project's contributing guide.
  2. Comment on the issue to say you are picking it up — it saves two people doing the same work.
  3. Fork the repository and make your change on a branch.
  4. Open a pull request that references the issue number.

Research direction

Start by reproducing the supplied nextflow run crisprseq command with count_table.count.txt, the MAGECK design matrix, and user.config. Trace the CNV-correction and MAGECK mle step to identify why execution takes a long time and fails; done means the same command completes successfully with CNV correction enabled.

Written by the indexing model from the issue text.

Assessment

Domain
bioinformatics
Issue type
Bug
Difficulty
4/5
Estimated time
3-5 days
Activity status
Stale
Clarity
Needs clarification
Newbie friendliness
25/100

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