nf-core / nf-core/createtaxdb

Consider adding a low-complexity sequence masking to genomes before building

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enhancement
Dominant language
Nextflow
Stars
22
Forks
13
Avg merge
8d 5h
Merged PRs (30d)
3

Description

Description of feature

This can in some cases improve classification as it doesn't try to find the best place for unspecific reads (assuming a tool accepts such masked genomes).

It may also make databases smaller as it will remove reundant unspecific regions (e.g. by reducing the number of kmers)

E.g. with dustmasker

Idea from: https://github.com/khyox/recentrifuge/wiki/Centrifuge-nt#step-by-step-instructions

Contributor guide

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First steps

  1. Read the whole issue, then the project's contributing guide.
  2. Comment on the issue to say you are picking it up — it saves two people doing the same work.
  3. Fork the repository and make your change on a branch.
  4. Open a pull request that references the issue number.

Research direction

No files, tests, or entry points are named in the issue. Start by locating the database-building step and reviewing how genomes are prepared before building; evaluate whether dustmasker is suitable for optional low-complexity masking. Done should include a defined masking workflow and clear evidence of its effect on classification and database size.

Written by the indexing model from the issue text.

Assessment

Domain
databases
Issue type
Feature
Difficulty
5/5
Estimated time
Over a week
Activity status
Stale
Clarity
Needs clarification
Newbie friendliness
25/100

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