Add IDR analysis
Nobody has claimed this yet.
- Dominant language
- Nextflow
- Stars
- 246
- Forks
- 184
- PR merge metrics
- No merged PRs in 30d
Description
Would be nice to add the IDR analysis that is currently carried out by the ENCODE pipelines:
https://github.com/kundajelab/atac-seq-pipeline
https://docs.google.com/document/d/1f0Cm4vRyDQDu0bMehHD7P7KOMxTOP-HiNoIvL1VcBt8/edit
Another implementation in NF:
https://github.com/DoaneAS/atacflow
ENCODE ATAC-seq Guidelines:
https://www.encodeproject.org/atac-seq/
Amongst other things, this will probably also involve creating pseudo-replicates by merging and equally sampling alignments across replicates.
Contributor guide
First steps
- Read the whole issue, then the project's contributing guide.
- Comment on the issue to say you are picking it up — it saves two people doing the same work.
- Fork the repository and make your change on a branch.
- Open a pull request that references the issue number.
Research direction
Start by reviewing the linked ENCODE ATAC-seq pipeline, atacflow implementation, ENCODE guidelines, and related nf-core/atacseq issue 36. Clarify the required IDR analysis and pseudo-replicate process, including merging and equally sampling alignments across replicates; done should include the requested analysis in the pipeline with appropriate validation.
Written by the indexing model from the issue text.
Assessment
- Domain
- bioinformatics
- Issue type
- Feature
- Difficulty
- 5/5
- Estimated time
- Over a week
- Activity status
- Stale
- Clarity
- Needs clarification
- Newbie friendliness
- 25/100