Latest version still generated MACS2, not MACS3
Nobody has claimed this yet.
- Dominant language
- Nextflow
- Stars
- 229
- Forks
- 141
- Avg merge
- 13h 41m
- Merged PRs (30d)
- 1
Description
Description of the bug
Hi,
I want to ask why I use latesest version, but my results is still macs2, not macs3.
Here is my command line:
nextflow run nf-core/atacseq -resume -c nextflow.config --input /project/sli68423_1316/users/yang/workspace/nanome/related_proje
ct/aml_nanopore/atac/00samplesheet_atac.csv --outdir results_mll_atac_hg38 -profile singularity --genome GRCh38 --read_length 50 -
-narrow_peak
Here is output directory:
ls bwa/merged_library/
ataqv D3_REP1.mLb.clN.sorted.bam D6_REP1.mLb.clN.sorted.bam deeptools
bigwig D3_REP1.mLb.clN.sorted.bam.bai D6_REP1.mLb.clN.sorted.bam.bai macs2
D1_REP1.mLb.clN.sorted.bam D4_REP1.mLb.clN.sorted.bam D7_REP1.mLb.clN.sorted.bam picard_metrics
D1_REP1.mLb.clN.sorted.bam.bai D4_REP1.mLb.clN.sorted.bam.bai D7_REP1.mLb.clN.sorted.bam.bai samtools_stats
D2_REP1.mLb.clN.sorted.bam D5_REP1.mLb.clN.sorted.bam D8_REP1.mLb.clN.sorted.bam
D2_REP1.mLb.clN.sorted.bam.bai D5_REP1.mLb.clN.sorted.bam.bai D8_REP1.mLb.clN.sorted.bam.bai
Command used and terminal output
nextflow run nf-core/atacseq -resume -c nextflow.config --input /project/sli68423_1316/users/yang/workspace/nanome/related_proje
ct/aml_nanopore/atac/00samplesheet_atac.csv --outdir results_mll_atac_hg38 -profile singularity --genome GRCh38 --read_length 50 -
-narrow_peak
Relevant files
No response
System information
No response
Contributor guide
First steps
- Read the whole issue, then the project's contributing guide.
- Comment on the issue to say you are picking it up — it saves two people doing the same work.
- Fork the repository and make your change on a branch.
- Open a pull request that references the issue number.
Research direction
Start by reproducing the reported command with the supplied configuration, profile, genome, and samplesheet, then inspect the bwa/merged_library/macs2 output. Trace which peak-calling component the workflow invokes and compare that behavior with the requested latest version; done means identifying the cause and confirming the expected MACS version is used.
Written by the indexing model from the issue text.
Assessment
- Domain
- bioinformatics
- Issue type
- Bug
- Difficulty
- 4/5
- Estimated time
- 3-5 days
- Activity status
- Stale
- Clarity
- Needs clarification
- Newbie friendliness
- 25/100