nf-core / nf-core/atacseq

Add single-cell analysis functionality

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enhancement
Dominant language
Nextflow
Stars
229
Forks
141
Avg merge
13h 41m
Merged PRs (30d)
1

Description

Should be possible to add a --single-cell parameter to the pipeline that processes the data accordingly. I would imagine that you just set the replicate id in the design file to 1 for all the samples. This will then merge all of the samples together to create a "bulk" sample to call the peaks. The data from the single-cell alignments will then need to be projected onto the merged peaks. Will need to look into the analysis downstream from that but the pipeline should already have most of the functionality.

Another pipeline written in Snakemake:
https://github.com/dbrg77/plate_scATAC-seq/blob/master/mSp_scATAC-seq/Snakefile

Other tools:
https://omictools.com/single-cell-atac-seq-analysis-category

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Research direction

Start by reviewing the pipeline and its design file, then compare the linked plate_scATAC-seq Snakefile for single-cell processing. Done means a --single-cell option supports merging samples for peak calling and projecting single-cell alignments onto the merged peaks, with downstream analysis addressed.

Written by the indexing model from the issue text.

Assessment

Domain
bioinformatics
Issue type
Feature
Difficulty
5/5
Estimated time
Over a week
Activity status
Stale
Clarity
Needs clarification
Newbie friendliness
20/100

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