Add single-cell analysis functionality
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- Dominant language
- Nextflow
- Stars
- 229
- Forks
- 141
- Avg merge
- 13h 41m
- Merged PRs (30d)
- 1
Description
Should be possible to add a --single-cell parameter to the pipeline that processes the data accordingly. I would imagine that you just set the replicate id in the design file to 1 for all the samples. This will then merge all of the samples together to create a "bulk" sample to call the peaks. The data from the single-cell alignments will then need to be projected onto the merged peaks. Will need to look into the analysis downstream from that but the pipeline should already have most of the functionality.
Another pipeline written in Snakemake:
https://github.com/dbrg77/plate_scATAC-seq/blob/master/mSp_scATAC-seq/Snakefile
Other tools:
https://omictools.com/single-cell-atac-seq-analysis-category
Contributor guide
First steps
- Read the whole issue, then the project's contributing guide.
- Comment on the issue to say you are picking it up — it saves two people doing the same work.
- Fork the repository and make your change on a branch.
- Open a pull request that references the issue number.
Research direction
Start by reviewing the pipeline and its design file, then compare the linked plate_scATAC-seq Snakefile for single-cell processing. Done means a --single-cell option supports merging samples for peak calling and projecting single-cell alignments onto the merged peaks, with downstream analysis addressed.
Written by the indexing model from the issue text.
Assessment
- Domain
- bioinformatics
- Issue type
- Feature
- Difficulty
- 5/5
- Estimated time
- Over a week
- Activity status
- Stale
- Clarity
- Needs clarification
- Newbie friendliness
- 20/100