nasa / nasa/GeneLab_Data_Processing

[Amplicon Illumina] Issue with datasets that have multiple assays of the same target

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Description

When running the pipeline from the DEV_Amplicon_Illumina_NF_conversion branch using datasets with multiple assays for the same target (ex. OSD-417), it is failing to create a runsheet (at GET_RUNSHEET process) with the following error:

Image

In addition, the --specify-runsheet argument cannot be passed to the pipeline as it is not part of the pipeline's declared parameters.

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Research direction

Start in the DEV_Amplicon_Illumina_NF_conversion branch under Amplicon/Illumina/Workflow_Documentation/NF_AmpIllumina-C and inspect the GET_RUNSHEET process. Reproduce the failure with dataset OSD-417 and check how the --specify-runsheet argument is declared. Done means the pipeline creates a runsheet for multiple assays of the same target and accepts the argument.

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Assessment

Domain
bioinformatics
Issue type
Bug
Difficulty
4/5
Estimated time
3-5 days
Activity status
Stale
Clarity
Mostly clear
Newbie friendliness
35/100

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