Opening multichannel tile scans results in tile rearrangement
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- Dominant language
- Java
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Description
imagej
I used a Ziess confocal to take a 3 channel tile scan and proceeded to stitch the tiles on Zen. Upon opening in ImageJ, I use hyperstack, spilt channels and stitch tiles (sometimes I leave this off since they're already stitched but the problem is the same either way). When the images load, they are distorted and only half the image appears with other tiles from the scan (some repeats) appearing in other parts of the window (see screenshot for example).
I've reinstalled the program (Windows 64) and nothing changed. The tiles arrange differently with each new image and some open just fine. I also tried this using the MacOS 64 version on my macbook and everything was fine.
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Research direction
Reproduce the multichannel tiled-scan workflow described: open the scan, use Hyperstack and Split Channels, and compare tile stitching on Windows 64 with the reported macOS behavior. Trace the image-loading and tile-arrangement path involved in those operations; done means the channels and tiles display in their correct positions without repeated or missing regions.
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Assessment
- Tech stack
- java
- Domain
- computer-vision, desktop
- Issue type
- Bug
- Difficulty
- 4/5
- Estimated time
- 3-5 days
- Activity status
- Stale
- Clarity
- Needs clarification
- Newbie friendliness
- 35/100