imagej / imagej/imagej2

Custom ROI set density faliure

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Dominant language
Java
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Description

When creating a custom ROI area after doing that automatically and measuring density, in different gels i get the same results while spots have the different density at visual evaluation. What can be solution for that?

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Research direction

Start by reproducing the reported workflow: create a custom ROI after automatic ROI selection, measure density, and compare results across gels with visibly different spot densities. Trace the ROI and density-measurement entry points in the Java project. Done means identifying why the measurements match and correcting the behavior, with a regression test for differing densities.

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Assessment

Tech stack
java
Domain
computer-vision
Issue type
Bug
Difficulty
4/5
Estimated time
3-5 days
Activity status
Stale
Clarity
Needs clarification
Newbie friendliness
25/100

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