galaxyproject / galaxyproject/training-material

Update required on "Inspection of a BAM file" section.

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sequence-analysis
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Description

https://github.com/galaxyproject/training-material/blob/53a15dbbd18b01c556eceb16c1e88dafdb9cbc7a/topics/sequence-analysis/tutorials/mapping/tutorial.md?plain=1#L189

Some discrepancies found when delivering a workshop using Mapping Tutorial.

1) Samtools stats (2.0.2+galaxy2).
- Use a reference sequence : Locally cached/Use a built-in genome (on the GTN) vs Locally cached ( correct version )
- Using genome : Mouse (Mus musculus): mm10 Full (on the GTN) vs Mouse (Mus musculus): mm10 ( correct version)

2) The output of the samtools stats before filtering
- The samtools output is not consistent with what's on the GTN.
- ~21,900 mismatches (on the GTN) vs 32663 ( actual output in the report)
- ~4,753,900 bases mapped (on the GTN) vs 5004018 ( actual output in the report)
- ~0.005 mismatches per mapped base (on the GTN) vs 6.527354e-03 ( actual output in the report)

3) The output of the samtools stats after filtering
- Before filtering: 95,412 reads and after filtering: 89,664 reads. (on the GTN)
- Reads mapped : 92152 and read properly paired 90644 ( actual output in the report) ??

Contributor guide

Open the contributing guide

Research direction

Open topics/sequence-analysis/tutorials/mapping/tutorial.md at the linked “Inspection of a BAM file” section and compare its Samtools stats settings and reported outputs with the workshop findings. Check the reference sequence, mm10 selection, and before- and after-filtering values. Done means the tutorial accurately reflects the current results and settings.

Written by the indexing model from the issue text.

Assessment

Tech stack
markdown
Domain
documentation
Issue type
Documentation
Difficulty
3/5
Estimated time
1-2 days
Activity status
Stale
Clarity
Mostly clear
Newbie friendliness
45/100

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