galaxyproject / galaxyproject/brc-analytics
scRNA-Seq workflow errors
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Description
in prep for a conference in early july, were trying to repro some plots from a paper. some issues have come up along the way
copying this from the chat where @scottcain posted it, to serve as a place to track any needed discussion or issues that arise out of it. can always close if none do.
> I'm putting on my user hat again... I'm trying to process a paired fastq set for T. brucie through a scRNA transcriptomics workflow and it errors out. The originating error (I think) is this on step 7: EXITING because of fatal ERROR: not enough memory for BAM sorting: SOLUTION: re-run STAR with at least --limitBAMsortRAM 29816090916 Jun 06 17:15:02 ...... FATAL ERROR, exiting
Here is the history:
https://usegalaxy.org/u/scottcain/h/t-brucie-cell-cycle
How do I do make this change (to use less memory) in the context of the workflow?
attn @mvdbeek
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