System doesn't calculate melting temperature for unsplit nucleotides if they are more then two
- Dominant language
- C++
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- 2d 11h
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Description
**Steps to Reproduce**
1. Go to Macromolecules mode - Flex canvas (empty)
2. Load from HELM: `RNA1{[2-damdA].[5hMedC].[Super-G].[AmMC6T].[Super-T].[5Br-dU]}|RNA2{[2-damdA].[2-damdA].[2-damdA].[5hMedC].[Super-G].[5Br-dU]}$RNA2,RNA1,6:pair-1:pair|RNA2,RNA1,5:pair-2:pair|RNA2,RNA1,4:pair-3:pair|RNA2,RNA1,3:pair-4:pair|RNA2,RNA1,2:pair-5:pair|RNA2,RNA1,1:pair-6:pair$$$V2.0`

3. Open Calculate properties (press `Alt+C`) and go to RNA/DNA tab
**Actual behavior**
Melting temperature is absent

**Expected behavior**
Melting temperature is `35.9`

AC=10
CG=10
GT=10
TT=5
TU=5
SP (strength parameter per base) = 8 ( sum of pair strength (40) divide by number of pairs (5))
L (length of nucleotide sequence) = 6
UPC = 0,14 M
NAC = 0,2 M
Melting temperature = `7,35 * 8+LN(17,34 * 6)+LN(4,96 * 0,14)+LN(0,89 * 0,2)-25,42` = `35,9340966`
As per [requirement](https://github.com/epam/Indigo/issues/2552):
> 3. The equation for the melting temperature is as follows:
> Tm [°C] = 7,35 * SP + 17,34 * ln(L) + 4,96 * ln(UPC) + 0,89 * ln(NAC) - 25,42
**Environment details:**
- Ketcher Version 3.4.0-rc.3 Build at 2025-05-15; 19:51:50
- Indigo Version 1.32.0-rc.2.0-g94ab270bd-wasm32-wasm-clang-19.0.0
- Chrome Version 136.0.7103.93 (Official Build) (64-bit)
- Win10
Found while testing: https://github.com/epam/ketcher/issues/5727
Contributor guide
No contributing guide indexed for this repository
Research direction
No source files or tests are named. Reproduce the HELM example in Macromolecules mode, open Calculate properties on the RNA/DNA tab, and trace the melting-temperature calculation for unsplit nucleotides; done means the reported value is 35.9°C and matches the stated equation.
Written by the indexing model from the issue text.
Assessment
- Tech stack
- cpp, wasm
- Domain
- backend
- Issue type
- Bug
- Difficulty
- 3/5
- Estimated time
- 1-2 days
- Activity status
- Stale
- Clarity
- Mostly clear
- Newbie friendliness
- 48/100