Can we use TITAN2 without having an environmental gradient ?

Hello,

I'm actually runing TITAN2 perfectly and I don't have any technical issus,but I would like to know if TITAN2 can be used and interpreted without a problem when we don't have an environmental gradient ?

My data is composed of multiple soil samples taken from two types of wastes, for these samples we run a 16S rRNA sequencing and have the concentrations of some environmental variables. These concentrations somtimes varies between 0.75 to 4.75 for the same environmental parameter in the different samples. So can we consider this variation as "gradient" and use TITAN2 in our data to show which taxa varies with concentrations of physicochemical parameters ?

Any confirmation or suggestion would be much appreciated,

Najoua

No files, tests, or entry points are mentioned. Start by examining TITAN2's assumptions about environmental gradients and the described 16S rRNA samples, including the two waste types and variable ranges. Done means documenting whether this use is valid and how the variation should be interpreted.

Written by the indexing model from the issue text.