deepcelllineage / deepcelllineage/mitolin
Align and visualize multiple (mitochondria) nucleotide sequence (.fasta) files
- Dominant language
- Jupyter Notebook
- Stars
- 2
- Forks
- 4
- PR merge metrics
- No merged PRs in 30d
Description
## Background
Looking at genetic alignments is a very important part of molecular biology. The human eye can pick up patterns on a small scale, then we can program the computer to pick out similar patterns on a much larger scale.
Making alignments creates an indexed location for each nucleotide in a fasta file. In turn, this will allow downstream processing like determining the distance between two sequences.
## Aim
Align the 9 .fasta files in this folder:
[mitolin/data/gen/nguyen_nc_2018/20190702-fastas-on-hpc/1739/20lines/](https://github.com/deepcelllineage/mitolin/tree/master/data/gen/nguyen_nc_2018/20190702-fastas-on-hpc/1739/20lines)
Related to [issue 5](https://github.com/deepcelllineage/mitolin/issues/5)
Related to [issue 1 fluHA](https://github.com/deepcelllineage/fluHA/issues/1)
## Method
You can start with Blast or Muscle. Muscle will generate a sequence distance score that can be used to make a lineage tree (aka dendogram/hierarchical clusters).
Wikipedia has a list of alignment visualization software [here](https://en.wikipedia.org/wiki/List_of_alignment_visualization_software).
## Document your work
Please fork & clone this repo. Check out a branch for your work, then push and make a PR for us to merge your note and files.
Add a note (can be .md or .ipynb) with your solution to [mitolin/nb](https://github.com/deepcelllineage/mitolin/tree/master/nb).
Your note should be named as follows:
- DATE-issue#-shortdescription.ext
e.g.:
- 20190701-i02-extract-chrM-fa.md
## Questions?
Please put questions related to this issue in this issue thread. If you want a quick response, post a link to your comment in this thread to Slack #deepcelllineage or DM @Deena. To join Slack enter your email address [here](http://bit.ly/JoinSlackFastaiSFbay). For questions NOT specifically related to this issue, get in touch through any of the communication methods listed in [DCL's overview README](https://github.com/deepcelllineage/overview/blob/master/README.md).
Contributor guide
No contributing guide indexed for this repository
Research direction
Start by inspecting the nine FASTA files in data/gen/nguyen_nc_2018/20190702-fastas-on-hpc/1739/20lines/ and the existing notebooks in nb. Compare the suggested Muscle or Blast approaches, align the sequences, and visualize the result. Done means a dated issue-7 note in nb documents the method and includes the alignment and visualization outputs.
Written by the indexing model from the issue text.
Assessment
- Tech stack
- jupyter-notebook
- Domain
- bioinformatics, data-visualization
- Issue type
- Feature
- Difficulty
- 4/5
- Estimated time
- 3-5 days
- Activity status
- Stale
- Clarity
- Mostly clear
- Newbie friendliness
- 35/100