is there any requirements to the expression matrix?
- Dominant language
- R
- Stars
- 14
- Forks
- 9
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Description
I'm very interested in finding such package for constrcuting ceRNA network.
I have read the paper for SPONGE theory where conduct construction of a pan-cancer ceRNA network with the data from UCSC, and expression matrix is performed a log2 transformation, as I known, the unit of the data in UCSC is log2(count+1), so I don't know whether there is some requirements to the expression matrix? Can I use normalized counts which is transformed via count(matrix, normalized = T)? or Can I use vst function from DESeq2 package to transform expression matrix?
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Research direction
No project file, test, or entry point is named. Start by reading the SPONGE input documentation and checking how its expression-matrix interface handles UCSC log2(count+1), normalized counts, and DESeq2 vst inputs. Done means documenting the accepted transformations and any relevant constraints.
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Assessment
- Tech stack
- r
- Domain
- bioinformatics
- Issue type
- Documentation
- Difficulty
- 3/5
- Estimated time
- 1-2 days
- Activity status
- Stale
- Clarity
- Needs clarification
- Newbie friendliness
- 20/100