daisybio / daisybio/SPONGE

is there any requirements to the expression matrix?

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Dominant language
R
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Description

I'm very interested in finding such package for constrcuting ceRNA network.
I have read the paper for SPONGE theory where conduct construction of a pan-cancer ceRNA network with the data from UCSC, and expression matrix is performed a log2 transformation, as I known, the unit of the data in UCSC is log2(count+1), so I don't know whether there is some requirements to the expression matrix? Can I use normalized counts which is transformed via count(matrix, normalized = T)? or Can I use vst function from DESeq2 package to transform expression matrix?

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Research direction

No project file, test, or entry point is named. Start by reading the SPONGE input documentation and checking how its expression-matrix interface handles UCSC log2(count+1), normalized counts, and DESeq2 vst inputs. Done means documenting the accepted transformations and any relevant constraints.

Written by the indexing model from the issue text.

Assessment

Tech stack
r
Domain
bioinformatics
Issue type
Documentation
Difficulty
3/5
Estimated time
1-2 days
Activity status
Stale
Clarity
Needs clarification
Newbie friendliness
20/100

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